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ID: 1063 Isolation and characterization of female germline stem cell derived from porcine ovary

Nguyen Huy Hoang 1
Nguyen Thi Bao Tran 1
Nguyen Van Thuan 2
Bui Hong Thuy 2, *

  1. Cell Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam
  2. Cell Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 70000, Vietnam
Correspondence to: Bui Hong Thuy, Cell Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 70000, Vietnam. Email: [email protected].
Volume & Issue: Vol. 4 No. S (2017): Abstract Proceeding: International conference INNOVATIONS IN CANCER RESEARCH AND REGENERATIVE MEDICINE 2017 | Page No.: S 145 | DOI: 10.15419/bmrat.v4iS.337
Published: 2017-09-05

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Copyright © 2017 by the author(s).

Copyright The Author(s) 2024. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

One of the most significant finding in stem cell area in the early 21st century is the founding of female germline stem cells (FGSCs). Establishment of FGSCs allowed new possibilities for the use of them in biotechnology and medicine. Hence, the purpose of this study was to establish, characterize the porcine female germline stem cells (pFGSCs) from porcine ovary. The result revealed the success in establishing pFGSCs from ovarian tissue. Most of the pFGSCs were round shape after in vitro culture, forming groups of cells that cluster around the ovarian cells colonies. Immunofluorescent analysis of pFGSCs showed that these cells expressed germ cell and stem cell markers such as: Vasa, Stella, c-kit and Oct4. After several weeks in in vitro culture, pFGSCs increased in number without the loss of proliferative potential. Our results suggested that pFGSCs isolated from adult mammalian ovary, under appropriate conditions, could undergo proliferation.

Keywords: Biology female germline stem cells immunofluorescent pFGSCs proliferation

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